Maia M., Monteiro F., Sebastiana M., Marques A.P., Ferreira A.E.N., Freire A.P., Cordeiro C., Figueiredo A. & Silva M.S. (2016) Metabolite extraction for high-throughput FTICR-MS-based metabolomics of grapevine leaves. EuPA Open Proteomics 12, 4-9. DOI: 10.1016/j.euprot.2016.03.002.NON-cE3c affiliated
In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. In any metabolic profiling study, the establishment of an effective metabolite extraction protocol is paramount. We developed an improved metabolite extraction method, compatible with DI-FTICR-MS-based metabolomics, using grapevine leaves. This extraction protocol allowed the extraction of polar and non-polar compounds, covering all major classes found in plants and increasing metabolome coverage.