2015) Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus.PeerJ, 3, e731. DOI:10.7717/peerj.731 (IF2015 2,183; Q1 Multidisciplinary Sciences) (
The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.