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Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus

  • Articles in SCI Journals
  • Aug, 2015

Sousa-Santos, C., Fonseca, P.J. & Amorim, M.C.P. (2015Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus

PeerJ, 3, e731. DOI:10.7717/peerj.731 (IF2015 2,183; Q1 Multidisciplinary Sciences)
Summary:

The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy-Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.


https://peerj.com/articles/731/